El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.

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Cultivo y fusion de protoplastos [1990]

Regeneration of fertile plants from Helianthus nuttalli and Helianthus giganteus L. Protoplasts fusion from S.

Potato, Solanum tuberosum L. A rapid calli proliferation was observed after colonies developed. Cytological and RAPD marker analysis confirm their hybrid nature. Somatic hybrids obtained by protoplast fusion protkplastos Solanum tuberosum L.

The ploidy of the prootoplastos used in plant regeneration has been shown to profoundly affect the protopplastos of variation observed in morphological characters and chromosome numbers among regenerant plants. Production of somatic hybrids by electrofusion in Solanum. Avoid resits and achieve higher grades with the best study guides, textbook notes, and class notes written by your fellow students. The authors would like to thank the International Potato Protoplastow for financial support and to Martha Williams de Castro who revised the English version of this article.


Protoplasts fusion and plant regeneration. Then, at fifty days, all culture media were replaced by a mOsm V-KM medium. Abstract Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L.

The enzyme treatment was carried out overnight with gentle agitation.

Protoplast fusion, culture and regeneration Prior to fusion, protoplasts from S. Shoot regeneration started twelve weeks after calli were transferred to MS medium. Plant Cell Tissue Organ Cult This study shows that somatic hybrid potato plants can be obtained by the fusion method presented.

The latter has a natural resistance to late blight, Phytophthora infestans Mont. In conclusion, somatic fusions between the cultivated potato Solanum tuberosum and the wild species S. A slot-blot hybridization method for screening somatic hybrids. Molecular characterization of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA RAPD markers. Non-transgenic applications of plant tissue culture in potato. For this analysis, DNA extraction xultivo performed according to Doyle and Doyle with some modifications.

Also available in bundle 1. Fusion-treated protoplasts were cultured in V-KM medium supplemented with bovine serum albumin.


Tema 9. Cultivo de protoplastos e hibridación somática – Tercero Biotecnología. – Stuvia

No generic book summaries, but the specific content you need to ace your exams. Production of somatic hybrids between frost – tolerant Solanum commersonii and Solanum tuberosum: RAPD and chromosomal analysis of the hybrids are also presented.

It has been demonstrated that protoplast-derived plants show a higher degree of chromosomal number variation than those derived from tissue or organ culture.

Materials and methods Plant material and protoplast isolation. Heterokaryons can not be identified because protoplasts of the parents were of the same type and shape. Everything you need to know about selling pritoplastos Stuvia.

Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L.

Isolamento e cultivo de protoplastos de porta-enxertos de citros

Ruiz de Galarreta y E. The same primer cultvo four specific bands of, and bp in S. With the study guides and notes written by fellow students, you are guaranteed to be properly prepared for your exams.

Segundo curso Primer curso.